Download >> Download Pcdf vector manual
Read Online >> Read Online Pcdf vector manual
cdf origin of replication
prsfduet-1
pcdfduet-1 vector
pcdf 1b plasmid
petduet-1
pacycduet
clodf13 origin
pcoladuet-1
3' Sequencing 2 Primer Sequence: GCTAGTTATTGCTCAGCGG. 5' Terminal: N-Term. 5' Terminal 2: N-Term. 3' Terminal: C-Term. 3' Terminal 2: C-Term. Tag 1: 6xHis. Tag 2: S15. Bacterial Resistance: Streptomycin. Notes: pCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two
cat gene (chloramphenicol resistance), and pCDFDuet-1 carries the CloDF13 replicon (12) and. aadA gene (streptomycin/spectinomycin resistance). Two kanamycin-resistant Duet vectors are available; pRSFDuet-1 carries the RSF1030 replicon (13, 14) and pCOLADuet-1 carries the ColA replicon (15). Each vector carries
pCDF cDNA Expression Lentivectors. Cat. #s CD100A-1 – CD111B-1. C. Map and Features for pCDF1-MCS2-EF1-copGFP Vector. Feature. Location*. Function. CMV/5'LTR. 1-415. Hybrid CMV promoter-R/U5 long terminal repeat; required for viral packaging and transcription gag. 762-1011. Packaging signal. RRE. 1012-
User Manual. I. Introduction and Background. A. Purpose of this Manual. This manual provides details and information necessary to generate expression constructs of your gene of interest in the pCDF lentivectors. Specifically, it provides critical instructions on amplification and cloning the cDNA into the pCDF Vectors, and.
This plasmid carries an origin derived from CloDF13 (1) and streptomycin/spectinomycin resistance, allowing for the option of coexpression with many other Novagen T7-based expression vectors. The vector contains a T7 promoter, lac operator, ribosome binding site (rbs), an amino terminal His•Tag® coding sequence,
pCDFDuet-1 Vector. pCDFDuet-1 sequence landmarks. T7 promoter-1. 3765–3781. T7 transcription start-1. 1. His•Tag® coding sequence. 83–100. Multiple cloning sites-1. (Nco I–Afl II). 69–168. T7 promoter-2. 214–230. T7 transcription start-2. 231. Multiple cloning sites-2. (Nde I–Avr II). 297–438. S•Tag™ coding sequence.
pCDF-1b carries an N-terminal His•Tag® coding sequence that is cleavable with enterokinase as well as an optional C-terminal S•Tag™ coding sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB392). Commercial use of this Product
pCDFDuet™-1 DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS; CoA; User Protocol; Citations; Vector Map; Vector Sequence. 71340: Price could not be retrieved: Minimum Quantity needs to be mulitiple of: Upon Order Completion More
pCDFDuet™-1. Bacterial vector with a CloDF13 (CDF) origin for the co-expression of two genes. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.
The pET-43.1 and pET-44 vectors are sold under patent license from University of Oklahoma for research use only. Licenses for Vectors containing the His•Tag® sequence are licensed under U. S. Patent Nos. 5,310,663 .. The pET, pRSF-2, and pCDF-2 Ek/LIC vectors are compatible with each other in the same cell and.
http://telegra.ph/Symantec-encryption-desktop-manual-11-25 http://qrcokns.forumchik.ru/viewtopic.php?id=154 http://weddingdancedirectory.com/m/feedback/view/Ap-masteries-season-4-guide https://bitbin.it/3FyvMmoO/ http://kzxmmer.bloggersdelight.dk/2017/11/25/finra-fingerprint-card-instructions/